Green Synthesis of Chitosan-Functionalized Zinc Oxide Nanoparticles - A Novel Antimicrobial Agent
Keywords:
Green synthesis, Chitosan-functionalized ZnO nanoparticles, Antimicrobial activity, Guava extractAbstract
This study presents a green synthesis method for Chitosan-functionalized zinc oxide (ZnO) nanoparticles using guava (Psidium guajava) leaf extract and Chitosan, a natural polymer derived from chitin. The eco-friendly approach leverages guava extract as a reducing and stabilizing agent and Chitosan to enhance nanoparticle stability and bioactivity. Synthesized ZnO nanoparticles exhibited a crystalline structure with sizes ranging from 14–28 nm, confirmed by XRD analysis, and a characteristic UV-Visible absorption peak at 335 nm. The antimicrobial activity of the nanoparticles was evaluated against E. coli, S. aureus, and S. enterica using a disc diffusion assay. Significant inhibition zones were observed, particularly at higher nanoparticle concentrations, indicating strong antibacterial potential. The mechanism involves oxidative stress induction and bacterial membrane disruption. This green synthesis approach provides an effective and sustainable alternative to combat antimicrobial resistance, aligning with green chemistry principles to minimize environmental impact.
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