Polymeric Micelles of Coriandrum Sativum Seed Oil – Preparation and In-Vitro Evaluation
Keywords:
Coriandrum sativum seed oil, Polymeric micelles, PEG 6000, Solvent diffusion technique.Abstract
Polymeric micelles are well known for their advantages like enhancement of bioavailability of poorly soluble drugs and herbal drug constituents. The present research work was aimed to prepare polymeric micelles of Coriandrum sativum seed oil (CSSO) and to evaluate them for in-vitro properties and antibacterial activity. Extraction of CSSO from Coriandrum sativum seed powder was performed by hydro distillation technique. Phytochemical evaluation of CSSO reveals the presence of tannins, alkaloids & saponins followed by the measurement of chemical indices and GC-MS analysis of CSSO. The polymeric micelles containing CSSO were prepared by solvent diffusion method using PEG 6000 as a polymer & Pluronic F127 as a stabilizer. The Box-Behnken design was employed considering drug & polymer concentration along with stirring time as independent variables and particle size, % entrapment efficiency and % cumulative drug release as the dependent variables. The optimized formulation had shown low CMC value, which indicates that it was stable. The particle size & zeta potential values were 220.5±0.21nm and -0.5±0.2 mV & % entrapment efficiency of 55.34%, % cumulative drug release of polymeric micelles after 24 hrs was found to be 70.86%. Drug-Excipient compatibility studies were carried out to find out the interactions between drug and other excipients. Phase contrast microscopy was employed to find out the morphology. FT-IR analysis had revealed that polymer & CSSO were compatible with each other & Morphology of the optimized formulation was spherical & well distributed. The in-vitro antibacterial studies using agar diffusion process had shown that the optimized polymeric micelles were found to be more effective against gram positive bacteria than gram negative bacteria.
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